Comprehensive CRISPR-Cas9 screen identifies important factors for plasmablast development

Discover efficient and targeted techniques to study the immune system

Date & Time

December 15th, 2022 | 2pm CET

Webinar Description

Through the production of highly specific antibodies, plasma cells (PCs) and their progenitors plasmablasts (PBs) are essential for acute and long-term host protection against infection. While several transcription factors are known to be essential for PC and PB differentiation and survival, many more remain to be elucidated.

To address this issue, Dr. Miriam Wöhner and co-workers at the Research Institute of Molecular Pathology (IMP) in Austria, performed targeted CRISPR-Cas9 screening of over 3,000 genes to assess their impact on PB development.

In this webinar you will:
  • Hear how an intelligent gene selection strategy delivered both breadth of relevant content and experimental efficiencies
  • Learn how ‘near physiological’ B cell differentiation was enabled in vitro using the iGB culture system
  • Find out about the 47 additional genes that are now known to be essential to PB development
  • Discover why Twist Oligo Pools are the perfect choice for high-throughput CRISPR knock-out studies



Webinar Registration

Speaker

Dr. Miriam Wöhner
PostDoc | Research Institute of Molecular Pathology (IMP)
After obtaining her PhD from Friedrich-Alexander University (FAU) in Erlangen, Germany, where she studied potential therapies for B cell leukemia, Miriam joined the lab of Professor Meinrad Busslinger at the Research Institute of Molecular Pathology (IMP) in Vienna, Austria. Here, she worked on a variety of transcription factors involved in plasma cell development, germinal center formation, and class-switch recombination. This work included a comprehensive 3,000-gene CRISPR-Cas9 screen to elucidate novel factors important for plasmablast development. In 2022, Miriam joined the lab of Professor Falk Nimmerjahn at FAU, where she is currently investigating the role of different macrophage subsets in metastasis development and growth.
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