CLUE — a simple, fast, and efficient approach for constructing custom sgRNA libraries

Date & Time

August 31st, 2021 | 8 am PDT (5 pm CEST)

Webinar Description

CRISPR/Cas9 and its many applications have gained increasing popularity in the scientific community and beyond. Many studies have utilized large, genome-wide screens designed to unravel gene functions in several contexts.

However, many high-complexity contexts, such as in vivo models or highly parallelized approaches using multiple different cell lines, are not readily amenable to genome-wide approaches. Accordingly, there is an increasing demand for custom sgRNA library design and construction to enable research in these technically challenging systems.

This webinar will introduce CLUE (custom library multiplexed cloning) — an easy-to-use bioinformatics and wet-lab workflow, allowing the generation of numerous high-quality, custom sgRNA libraries.

In this webinar you will:
  • Discover how CLUE can be used to clone multiple custom sgRNA libraries from a single oligo pool
  • Learn about the cost and efficiency benefits of multiplexed, focused libraries
  • Explore the simple online tool for easy and fast oligo pool design
  • See examples where this approach, combined with a robust cloning procedure, has produced high-quality sgRNA libraries with highly uniform distributions

Webinar Registration

Dr. med. Christian Braun

Group Leader and Resident Physician in Pediatrics
University Hospital of Munich (LMU), Germany

Christian attained his medical degree at the University of Göttingen, Germany. He has a long-standing interest in functional genomics and in deciphering the systematic rewiring of cancer transcriptomes. After he completed his postdoctoral training with Michael Hemann at MIT in the US, he returned to Germany to start his own lab at LMU Munich’s University Hospital in 2017. Since 2019, his lab has been funded by the Max Eder Program — a set up grant awarded by German Cancer Aid. Christian is driven by the idea of molecular target discovery for cancer therapy and the translation of his findings into clinical applications. This passion is reinforced on a daily basis through his work as a senior resident physician in pediatrics, where he treats children suffering from devastating diseases.

Dr. Martin Becker

Head of NewTechnology
AVA LifeScience GmbH

Martin studied biotechnology in Braunschweig, Germany and cell and molecular biology in Rhode Island, USA. He subsequently obtained a PhD in Immunology from the Max Planck Institute of Immunology and Epigenetics in Freiburg, Germany. After successful completion of his PhD, Martin moved to Munich, Germany, joining the Helmholtz Zentrum to work on the establishment of a CRISPR/Cas9 screening pipeline in patient-derived xenograft models of acute leukemias. Within this project, Martin and Christian joined forces and established a multiplex cloning protocol for custom sgRNA libraries named CLUE. In 2020, Martin was recruited by AVA LifeScience to lead their R&D team, developing novel standards for FACS-based diagnostics.
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