Scalable and combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing

High-throughput, high-resolution analysis of genetic perturbations and interactions

Webinar Details

October 6th, 6 PM PDT | October 7th, 9 AM SGT

Webinar Description

The recent advent of genetic tools, such as Peturb-seq, that combine the analysis of pooled CRISPR screens with single-cell RNA sequencing has delivered unprecedented insights into mammalian gene function and genetic regulatory networks. However, current implementations of these tools face technical and practical limitations that restrict their use.

To overcome these challenges, researchers at University of California - San Francisco, Princeton University, and 10x Genomics have developed “direct-capture” Peturb-seq, which enables substantially higher throughput investigations into genetic interactions. Furthermore, through the addition of a hybridization-based target enrichment methodology, sensitive and specific sequencing of biologically meaningful panels of transcripts is now possible — significantly reducing experimental costs.


In this webinar you will…
  • Learn how Perturb-seq, which combines pooled CRISPR screens with single-cell RNA-sequencing, facilitates unbiased exploration of gene function and delineation of genetic regulatory networks.
  • Hear about the recent innovation of direct-capture Perturb-seq, which significantly increases screening throughput – substantially expanding the scale and flexibility of this technology.
  • Understand how targeting individual genes with multiple sgRNAs per cell improves efficacy of CRISPR interference and activation, facilitating the use of compact, highly active CRISPR libraries for single-cell screens.
  • See how hybridization-based target enrichment can decrease sequencing costs for transcripts of interest by up to 90%.
  • Discover how the scalability and accuracy of Twist’s CRISPR Oligo Pools and NGS Target Enrichment solutions supported the development and application of this ground-breaking technology.
Webinar Registration

Speaker

Joseph Replogle
University of California San Francisco

Joseph Replogle is a UCSF MD/PhD student in Jonathan Weissman's lab working on single-cell CRISPR screens with the goal of bringing high-resolution functional genomics to diverse primary cell types. Before medical school, Joseph worked in Philip De Jager and Barbara Stranger's labs at Harvard Medical School and the Broad Institute studying the genetics of multiple sclerosis and Alzheimer's disease. He received his undergraduate degree in Molecular Biology at Pomona College.
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