Webinar Description

First published in 2019, the technique of prime editing offers significant advantages over other genome editing methods, such as CRISPR/Cas9. One critical benefit is the introduction of single-strand, rather than double-strand breaks in the target DNA, which allows the more accurate mismatch repair mechanism to be deployed — reducing the number of unwanted and erroneous edits. In addition, prime editing can install a wider range of edits, including transitions (interchanges of purines or of pyrimidines) and transversions (purines to pyrimidines, and vice versa), as well as deletions and insertions.

Despite its promise, the factors that govern the successful integration of short sequences to a genomic target using prime editing remain unclear.
In this webinar, researchers from the Wellcome Sanger Institute, UK, will describe their ground-breaking work analyzing thousands of insertion sequences in different genomic contexts to understand and predict prime editing insertion efficiencies.

In this webinar you will:

Discover the impact of sequence length, composition, and secondary structure on prime editing insertion rates
Hear how the 3' flap nucleases TREX1 and TREX2 suppress the insertion of longer sequences
Learn about MinsePIE, a free web application for accurate prediction of insertion efficiency
Find out how high-quality Twist Oligo Pools supported this cutting-edge research

Speakers

Juliane Weller

PhD Student | Parts Group, Wellcome Sanger Institute

Juliane Weller, a third-year PhD student in the laboratory of Dr. Leopold Parts at the Wellcome Sanger Institute, UK, uses paired CRISPR prime editing to introduce genetic variants, including deletions, in vitro. To better understand the determinants of prime editing efficiency and guide experimental design, Juliane has been building predictive models for short sequence insertions based on editing efficiency rates of thousands of short sequences from pooled CRISPR prime editing screens at different target sites and in different cell lines.

Jonas Koeppel

PhD Student | Parts Group, Wellcome Sanger Institute

Jonas Koeppel is a fourth-year PhD student in the laboratory of Dr. Leopold Parts at the Wellcome Sanger Institute, UK. Jonas uses CRISPR prime editing to insert recombinase recognition sequences into the genomes of mammalian cells to trigger large-scale genome rearrangements. To further develop the prime editing technology and build predictive models to guide the choice of optimal reagents, he characterized the insertion efficiencies for thousands of short sequences using pooled CRISPR prime editing screens in different cell lines and repair contexts.

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Guannan Wang, PhD, Senior Research Investigator, discusses how they are identifying the driver mutations and clinically actionable biomarkers in all common canine cancers, to arm veterinary clinicians with better prognostic information, target therapeutic options, and markers to predict treatment response, ultimately enabling precision medicine in canine oncology.

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Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants