Eliminate Bias and Undesirable Motifs

- Effortlessly incorporate multiple variant domains into one or multiple scaffolds
- Precise control over codon usage (all 64 codons), amino acid distribution, and length variation
- Rigorous quality control, including NGS verification of modified regions
- Sequence variant ratios documented
- Uniform distribution of user-defined variants
- No premature stop codons or unwanted codons
- Design all sequences, single or multiple domains and combinations - single, pairwise, or triple variants
- Modular synthesis system enables iteration of future libraries
- Greater flexibility than TRIM and the degenerate approach
Twist’s massively parallel silicon-based DNA synthesis platform produces highly uniform and accurate oligos, with 90% of oligos represented within <2.5x of the mean, along with an industry-leading low error rate of 1:2,000 nt.
Combined with our well established molecular biology expertise, the Twist oligo synthesis platform enables the fabrication of highly diverse gene mutant libraries with excellent variant representation and highly specific user-defined composition with no unwanted bias or motifs. Twist library technology enables a comprehensive interrogation of the variant sequence space.
For now until 31 March, we are offering 50% off subsequent clonal genes order of up to an order value of the original CVL purchase.
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