The complex patterns of gene expression that enable multi-cellularity and cell differentiation during animal development are encoded in the sequences of gene promoter and enhancer regions, which are bound by transcription factor (TF) and co-factor proteins.
Until recently, knowledge of the intricate nature of these interactions was limited to the study of a small number of enhancers in specific cell types and conditions. However, the advent of massively parallel reporter assays (MPRAs) coupled with access to high numbers of high-quality, synthetic DNA oligonucleotides is now providing deep insights into these relationships.
Join this webinar to hear Dr. Cosmas Arnold and Bernardo Almeida from the Research Institute for Molecular Pathology (IMP), Austria, share their latest research on transcriptional regulation.
In this webinar you will:
- Learn how systematic mutagenesis of enhancer TF motifs coupled with STARR-seq revealed the importance of specific motif combinations for enhancer function
- Discover how promoter-cofactor compatibility can be elucidated using synthetic promoter candidates combined with STAP-seq
- Hear how different types of promoters are differentially activated by distinct cofactors
- Find out how Twist oligo pools can support transcriptional regulation studies — providing new insights into functional genomics
