Decoding transcriptional regulation using massively parallel reporter assays and Twist Oligo Pools

The latest advances in studying gene expression regulation

Date & Time

April 14th, 2021 | 8 am PST (5 pm CEST)

Webinar Description

The complex patterns of gene expression that enable multi-cellularity and cell differentiation during animal development are encoded in the sequences of gene promoter and enhancer regions, which are bound by transcription factor (TF) and co-factor proteins.

Until recently, knowledge of the intricate nature of these interactions was limited to the study of a small number of enhancers in specific cell types and conditions. However, the advent of massively parallel reporter assays (MPRAs) coupled with access to high numbers of high-quality, synthetic DNA oligonucleotides is now providing deep insights into these relationships.

Join this webinar to hear Dr. Cosmas Arnold and Bernardo Almeida from the Research Institute for Molecular Pathology (IMP), Austria, share their latest research on transcriptional regulation.

In this webinar you will:
  • Learn how systematic mutagenesis of enhancer TF motifs coupled with STARR-seq revealed the importance of specific motif combinations for enhancer function
  • Discover how promoter-cofactor compatibility can be elucidated using synthetic promoter candidates combined with STAP-seq
  • Hear how different types of promoters are differentially activated by distinct cofactors
  • Find out how Twist oligo pools can support transcriptional regulation studies — providing new insights into functional genomics
Webinar Registration

Dr. Cosmas Arnold

Research Associate
Research Institute for Molecular Pathology (IMP)

Cosmas is a molecular biologist with great interest in gene regulation, functional genomics, and technology development. He received his PhD in 2013 and currently holds a position as Research Associate in the lab of Dr. Alexander Stark at the Research Institute of Molecular Pathology (IMP) in Vienna, Austria. During his scientific career, he has developed several novel technologies to study gene regulation, including STARR-seq and STAP-seq.

Bernardo Almeida

PhD Student
Research Institute for Molecular Pathology (IMP)

Bernardo Almeida is a PhD student in Dr. Alexander Stark’s lab, where he is using computational approaches to understand the cis-regulatory information encoded in the sequence of transcriptional enhancers. He received his undergraduate degree in Biomedical Sciences and MSc in Oncobiology at the University of Algarve, Portugal. During his master thesis with Nuno Barbosa Morais at Instituto de Medicina Molecular (IMM, Portugal), he studied the genetics and molecular mechanisms underlying tumor development.
©. All Rights Reserved Twist Bioscience.