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エクソームシーケンシングにおいて、ターゲットエンリッチメントプローブパネルの設計と生成は、キャプチャ効率に固有の影響をもたらします。この資料では、市販のエクソームキットによく見られる広範なオンターゲット率と均一性を用いて、実験効率全体に対する両メトリクスの相対的影響を数学的にモデル化します。市販のプローブパネルのほとんどは、仕様としてオンターゲット率のみ示していますが、ターゲットを絞ったシーケンシング実験の効率およびオーバーサンプリング要件を大きく左右するのは、実は均一性です。

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Simple pricing

Gene Fragments*

Clonal Genes*

AVERAGE LENGTH

0.3kb
0.6kb
0.9kb
1.2kb
1.5kb
1.8kb

0.3kb
1kb
2kb
3kb
4kb
5kb

base pairs

Please enter a quantity between 300 and 1800.

base pairs

Please enter a quantity between 300 and 5000.

QUANTITY

genes

Please enter a quantity between 1 and 10000.

ESTIMATED TOTAL

Based on cloning in a Twist vector

$10,500

TWIST CLONAL GENES WILL SAVE YOU AN ESTIMATED**

TWIST SAVES YOU AN ESTIMATED**

$90,000on reagents & kits

BREAKDOWN

Vector prep

$45

Amplification

$35

Cloning

$60

$40

TOTAL

~ $180 per gene

36 hrsof manual cloning

Manual cloning takes about 6 hours per 96 genes

ORDER NOW

SAVINGS WITH TWIST
COMPARED TO***

NaN%

SAVINGS

*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.
**Cost savings based on comparison to public list prices for standard reagents. Time savings based on Twist's internal testing of typical times for standard manual cloning.
***Savings based on comparison to competitor’s publicly-listed pricing as of May 15, 2019. Turn-around times between Twist and competitors may vary.

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I am VERY happy with Twist and the fact that they came on scene right when I needed them. I have recommended the company to everyone I know who requires gene fragments or DNA for cloning.

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I am VERY happy with Twist and the fact that they came on scene right when I needed them. I have recommended the company to everyone I know who requires gene fragments or DNA for cloning.

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I am VERY happy with Twist and the fact that they came on scene right when I needed them. I have recommended the company to everyone I know who requires gene fragments or DNA for cloning.

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