Accurate fragment synthesis accelerates discovery. Twist Gene Fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones.
In a direct comparison with competitive Gene Fragment products, Twist outperformed with the lowest error rate.
The same sequences were ordered from Twist and Integrated DNA Technologies, Inc. (IDT) for genes of various lengths, and the genes were then cloned and transformed. Colonies were then picked and sequenced using NGS. The data represent the average error rate across the given sequences in each set for Twist Gene Fragments, eBlocks, and gBlocks. The results indicate an average of 2-fold reduction in error rate, or 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over gBlocks.
Lower error rates improve your cloning process by minimizing colony screening. With Twist Gene Fragments you should expect to pick 1-2 colonies even for longer genes. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.